The utility model relates to a cow womb content sampler. The cow womb content sampler comprises a collecting tube. A front end cap and a back end cap are respectively arranged at the front end and the back end of the collecting tube, wherein the front end cap is provided with a content hole, and the back end cap is provided with a threaded hole. A front piston and a back piston are arranged in the collecting tube. The back piston is connected with a threaded rod which penetrates through the threaded hole. One end portion of the threaded rod is provided with a handle. According to the cow womb content sampler, the front end of the collecting tube is provided with the front end cap so as to effectively prevent vagina secreta from entering the collecting tube when the sampler passes through the vagina, meanwhile, collected womb content is always in the collecting tube so that when passing through the vagina the sampler can not be stained with pathogenic bacteria in the vagina just like a cotton swab, and therefore probability of secondary pollution is reduced, accuracy of detection results is improved, and the probability of erroneous judgment of the detection results is reduced.